Little Group Protocols
- Digoxigenin-labelled Riboprobe Synthesis from a PCR-generated DNA template
- Semi-Automated Section in situ Hybridisation for 7µm paraffin sections
- Semi-Automated Whole Mount in situ Hybridisation
- Immunohistochemistry on paraffin sections
Rumballe BA, Chiu HS, Georgas KM and Little MH.
Use of in situ hybridization to examine gene expression in the embryonic, neonatal and adult urogenital system.
Rumballe B, Georgas K, Little MH.
Georgas K, Rumballe B, Wilkinson L, Chiu HS, Lesieur E, Gilbert T, Little MH.
Use of dual section mRNA in situ hybridisation/immunohistochemistry to clarify gene expression patterns during the early stages of nephron development in the embryo and in the mature nephron of the adult mouse kidney.